당뇨 유도 백서 경골에 매식한 티타늄 임플란트 주위에 지방조직 유래 줄기세포 적용 시 골치유에 미치는 영향
Effect of adipose-derived stem cells on bone healing on titanium implant in tibia of diabetes mellitus induced rats
김민구, 정인교, 신상훈, 김철훈, 김복주, 김정한, 황영섭, 정유진, 김진우, 김욱규,
소속 상세정보
김민구 ( Kim Min-Gu ) - 동아대학교 의과대학 구강악안면외과학교실
정인교 ( Jung In-Kyo ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
신상훈 ( Shin Sang-Hun ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김철훈 ( Kim Chul-Hoon ) - 동아대학교 의과대학 구강악안면외과학교실
김복주 ( Kim Bok-Joo ) - 동아대학교 의과대학 구강악안면외과학교실
김정한 ( Kim Jung-Han ) - 동아대학교 의과대학 구강악안면외과학교실
황영섭 ( Hwang Young-Sup ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
정유진 ( Jung Eu-Gene ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김진우 ( Kim Jin-Woo ) - 동아대학교 의과대학 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0355620100360050392
Abstract
Introduction: Diabetes mellitus, as a major health problem for the elderly has been shown to alter the properties of the bone and impair bone healing around a titanium implant in both humans and animals. The aim of this study was to examine the effect of adipose-derived stem cells on the healing process around a titanium implant in streptozotocin-induced diabetic rats.
Materials and Methods: Thirteen rats were divided into two groups: adipose-derived stem cells injected group and a control group. A titanium screw implant (diameter: 2.0 ㎜, length: 3.5 ㎜) was placed into both tibia of 13 rats: 13 right tibia as the control group and 13 left tibia as the experimental group. The rats were sacrificed at different intervals (1, 2, and 4 weeks) after implantation for histopathology observations and i㎜unohistochemistric analysis.
Results: The histopathological findings revealed earlier new formed bone in the experimental group than the control group. In particular, at 1 week after implantation, the experimental group showed more newly formed bone and collagen around the implant than the control group. In i㎜unohistochemistric analysis, osteoprotegerin (OPG) expression in the experimental group increased early compared to that of the control group until 2 weeks after implantation. However, after 2 weeks, OPG expression in the experimental group was similar to OPG expression in the control group. The receptor activator of nuclear factor κB ligand (RANKL) expression in the experimental group increased early compared to that of the control group, and then decreased at 2 weeks. After 2 weeks, the level of RANKL expression was similar in both groups.
Conclusion: These results suggest that adipose-derived stem cells in implantation can promote bone healing around titanium, particularly in diabetes mellitus induced animals.
키워드
Stem cell;Dental implants;Diabetes mellitus;Rats
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